Composite

Part:BBa_K2868000:Design

Designed by: Cale Lester   Group: iGEM18_Stanford-Brown-RISD   (2018-07-30)


T7 csgA His-tagged


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 513
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence was optimized for E.coli using the IDT optimization tool. Some codons were edited away from the optimal E.coli codon in order to avoid iGEM illegal cut sites. The standard "CAT" repeats for his-tag were diversified into both "CAT" and "CAC" on advice from our mentor.


Source

The T7 promoter and RBS and terminator all originate from the T7 bacteriophage. The csgA coding sequence originates from E.coli.

References